Genetic Transformation of Streptococcus mutans
نویسندگان
چکیده
منابع مشابه
Genetic transformation of Streptococcus mutans.
Three strains of Streptococcus mutans belonging to serotypes a, c, and f were transformed to streptomycin resistance by deoxyribonucleic acids derived from homologous and heterologous streptomycin-resistant strains of S. mutans and Streptococcus sanguis strain Challis. Homologous transformation of S. mutans was less efficient than heterologous transformation by deoxyribonucleic acids from other...
متن کاملGenetic transformation of putative cariogenic properties in Streptococcus mutans.
Rough colonial morphology and bacteriocin production, two properties which may be associated with the cariogenicity of Streptococcus mutans, were transformed into several strain GS-5 mutants defective in each respective property. Transformation was determined by observing the frequency of cotransfer of these properties with different reference markers. The rough colonial transformants were iden...
متن کاملNatural genetic transformation of Streptococcus mutans growing in biofilms.
Streptococcus mutans is a bacterium that has evolved to be dependent upon a biofilm "lifestyle" for survival and persistence in its natural ecosystem, dental plaque. We initiated this study to identify the genes involved in the development of genetic competence in S. mutans and to assay the natural genetic transformability of biofilm-grown cells. Using genomic analyses, we identified a quorum-s...
متن کاملGenetic regulation of fructosyltransferase in Streptococcus mutans.
Streptococcus mutans possesses several extracellular sucrose-metabolizing enzymes which have been implicated as important virulence factors in dental caries. This study was initiated to investigate the genetic regulation of one of these enzymes, the extracellular fructosyltransferase (Ftf). Fusions were constructed with the region upstream of the S. mutans GS5 Ftf gene (ftf) and a promoterless ...
متن کاملDevelopment of competence for genetic transformation of Streptococcus mutans in a chemically defined medium.
Streptococcus mutans develops competence for genetic transformation in response to regulatory circuits that sense at least two peptide pheromones. One peptide, known as CSP, is sensed by a two-component signal transduction system through a membrane receptor, ComD. The other, derived from the primary translation product ComS, is thought to be sensed by an intracellular receptor, ComR, after upta...
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ژورنال
عنوان ژورنال: Infection and Immunity
سال: 1981
ISSN: 0019-9567,1098-5522
DOI: 10.1128/iai.32.3.1295-1297.1981